A novel gyrB gene mutation in fluoroquinolone resistant clinical isolates of Mycobacterium tuberculosis
نویسندگان
چکیده
Methods A total of 100 ofloxacin resistant (OFX r) and 100 OFX sensitive (OFX s) isolates of M.tuberculosis isolates were consecutively selected from routine TB laboratory during 2012-2013. All the isolates were screened for phenotypic OFX r (>2μg/ml) by 1% proportion method and tested for minimal inhibitory concentration by absolute concentration method. Quinolone resistance determining region (QRDR) of gyrA and gyrB genes of 320bp and 428bp respectively were amplified, sequenced and compared with M.tuberculosis H37Rv.
منابع مشابه
High proportion of fluoroquinolone-resistant Mycobacterium tuberculosis isolates with novel gyrase polymorphisms and a gyrA region associated with fluoroquinolone susceptibility.
Fluoroquinolone resistance in Mycobacterium tuberculosis can be conferred by mutations in gyrA or gyrB. The prevalence of resistance mutations outside the quinolone resistance-determining region (QRDR) of gyrA or gyrB is unclear, since such regions are rarely sequenced. M. tuberculosis isolates from 1,111 patients with newly diagnosed culture-confirmed tuberculosis diagnosed in Tennessee from 2...
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عنوان ژورنال:
دوره 14 شماره
صفحات -
تاریخ انتشار 2014